Mitochondrial Dna Methods And Protocols Pdf

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mitochondrial dna methods and protocols pdf

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This form of genetic analysis can be applied to human, animal, and plant specimens.

Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial DNA relative to the nuclear DNA levels provides important information on potential mitochondrial toxicity. All of the work was sponsored by Gilead Sciences, Inc.

Nutrient status shapes selfish mitochondrial genome dynamics across different levels of selection

This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. In most mammalian cells, mitochondria generate the bulk of ATP required to sustain a plethora of diverse cellular processes. Besides generating ATP, mitochondria also play important roles in intracellular calcium signalling [ 1 ], apoptosis [ 2 ], reactive oxygen species ROS production [ 3 ], biosynthesis of heme and iron-sulphur clusters [ 4 , 5 ], and other cellular processes.

Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. Stuart Published Biology. Bogenhagen 2. Gulliver, and Ian J.

It seems that you're in Germany. We have a dedicated site for Germany. This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration. JavaScript is currently disabled, this site works much better if you enable JavaScript in your browser. Biomedical Sciences Human Genetics.

Determination of Mitochondrial DNA Upon Drug Treatment

Mitochondrial DNA copy number mtDNA-CN , a measure of the number of mitochondrial genomes per cell, is a minimally invasive proxy measure for mitochondrial function and has been associated with several aging-related diseases. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: Data from this study are available upon request as these data contain potentially identifying and sensitive patient information. Rotter and Dr. The funding organizations had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication. Competing interests: The authors have declared that no competing interests exist.

Mitochondrial DNA : methods and protocols

The Human Mitochondrial Genome: From Basic Biology to Disease offers a comprehensive, up-to-date examination of human mitochondrial genomics, connecting basic research to translational medicine across a range of disease types. Here, international experts discuss the essential biology of human mitochondrial DNA mtDNA , including its maintenance, repair, segregation, and heredity. Furthermore, mtDNA evolution and exploitation, mutations, methods, and models for functional studies of mtDNA are dealt with. Disease discussion is accompanied by approaches for treatment strategies, with disease areas discussed including cancer, neurodegenerative, age-related, mtDNA depletion, deletion, and point mutation diseases.

It seems that you're in Germany. We have a dedicated site for Germany. Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve.

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Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition.


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    This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial.

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