Systems Biology Of Stem Cell Fate And Cellular Reprogramming Pdf

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systems biology of stem cell fate and cellular reprogramming pdf

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Metrics details. Heterogeneous gene expressions of cells are widely observed in self-renewing pluripotent stem cells, suggesting possible coexistence of multiple cellular states with distinct characteristics. Though the elements regulating cellular states have been identified, the underlying dynamic mechanisms and the significance of such cellular heterogeneity remain elusive.

Systems biology of stem cell fate and cellular reprogramming

The application of Systems Biology approaches to Stem Cell research is becoming more and more necessary in order to address a variety of fundamental questions in this field. In particular, the identification of optimal sets of genes, whose perturbations can trigger specific cellular transitions, remains a challenge in the application of cellular reprogramming and differentiation strategies to regenerative medicine. In this talk, I will present computational approaches developed in our lab, which are based on cellular network models for the identification of cellular transition-dependent reprogramming and cell fate determinant genes. Our computational predictions have been experimentally validated in different cellular systems. Furthermore, this novel approach systematically makes these predictions without prior knowledge of potential candidate genes and pathways involved, providing guidance on systems where little is known. In addition, we recently implemented a computational method that integrates cellular signaling and gene regulatory networks to identify key signaling pathways controlled by the niche to maintain specific cellular phenotypes, and whose perturbations could trigger cellular transitions in-vivo.

Cellular reprogramming has been recently intensively studied experimentally. We developed a global potential landscape and kinetic path framework to explore a human stem cell developmental network composed of 52 genes. We uncovered the underlying landscape for the stem cell network with two basins of attractions representing stem and differentiated cell states, quantified and exhibited the high dimensional biological paths for the differentiation and reprogramming process, connecting the stem cell state and differentiated cell state. Both the landscape and non-equilibrium curl flux determine the dynamics of cell differentiation jointly. Flux leads the kinetic paths to be deviated from the steepest descent gradient path, and the corresponding differentiation and reprogramming paths are irreversible. Quantification of paths allows us to find out how the differentiation and reprogramming occur and which important states they go through.

Julio C. Pluripotent stem cells are regarded as a promising cell source to obtain human dopamine neurons in sufficient amounts and purity for cell replacement therapy. Importantly, the success of clinical applications depends on our ability to steer pluripotent stem cells towards the right neuronal identity. In Parkinson disease, the loss of dopamine neurons is more pronounced in the ventrolateral population that projects to the sensorimotor striatum. Because synapses are highly specific, only neurons with this precise identity will contribute, upon transplantation, to the synaptic reconstruction of the dorsal striatum. Thus, understanding the developmental cell program of the mesostriatal dopamine neurons is critical for the identification of the extrinsic signals and cell-intrinsic factors that instruct and, ultimately, determine cell identity.


Understanding and engineering chromatin as a dynamical system. In press at Cell Systems. Wang, N. Beitz, A, and Galloway, KE. Engineering cell fate: Applying synthetic biology to cellular reprogramming. In press at Current Opinion in Systems Biology.

Nanog induced intermediate state in regulating stem cell differentiation and reprogramming

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Stem Cell Res. Online ahead of print. The pioneer and differentiation factor FOXA2 is a key driver of yolk-sac tumour formation and a new biomarker for paediatric and adult yolk-sac tumours. J Cell Mol Med. A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference.

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Human pluripotent stem cells hold great promise for developments in regenerative medicine and drug design. The mathematical modelling of stem cells and their properties is necessary to understand and quantify key behaviours and develop non-invasive prognostic modelling tools to assist in the optimisation of laboratory experiments. Here, the recent advances in the mathematical modelling of hPSCs are discussed, including cell kinematics, cell proliferation and colony formation, and pluripotency and differentiation. Human pluripotent stem cells hPSCs have the ability to self-renew indefinitely through repeated divisions mitosis and can differentiate into any bodily cell type the pluripotency property. The latter property underpins their promising clinical applications in drug discovery, cell-based therapies and personalised medicine [ 1 , 2 ].

Смит откашлялся. - Халохот ликвидировал его с помощью НТП - непроникающей травматической пули. Это резиновая капсула, которая при попадании растворяется. Все тихо и чисто. Перед сердечным приступом мистер Танкадо не почувствовал ничего, кроме легкого укола.

Код ошибки 22. Она попыталась вспомнить, что это. Сбои техники в Третьем узле были такой редкостью, что номера ошибок в ее памяти не задерживалось. Сьюзан пролистала справочник и нашла нужный список. 19: ОШИБКА В СИСТЕМНОМ РАЗДЕЛЕ 20: СКАЧОК НАПРЯЖЕНИЯ 21: СБОЙ СИСТЕМЫ ХРАНЕНИЯ ДАННЫХ Наконец она дошла до пункта 22 и, замерев, долго всматривалась в написанное. Потом, озадаченная, снова взглянула на монитор.

in presentations that covered stem cell heterogeneity, cell fate decision-making systems biological approaches for achieving a better understanding of stem cell and by nuclear reprogramming experiments (Eggan et al., ;. Hochedlinger mechanisms of cellular state transitions, and in addition to the development.

Stem Cells International

 - Во множестве шифров применяются группы из четырех знаков. Возможно, это и есть ключ. - Вот именно, - простонал Джабба.  - Он над вами издевается. А вы тем временем погибаете.  - Он посмотрел на экран.  - Осталось девять минут.

Она превозмогала боль сколько могла, но ее силы иссякли. Она сказала ему, что их брак исчерпал себя, что она не собирается до конца дней жить в тени другой женщины. Вой сирен вывел его из задумчивости. Его аналитический ум искал выход из создавшегося положения. Сознание нехотя подтверждало то, о чем говорили чувства.


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    Stem cell differentiation and the maintenance of self-renewal are intrinsically complex processes. They require the coordinated and dynamic.

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